Composite
PBad LuxI

Part:BBa_K3484005:Design

Designed by: Tomas Berjaga, Quim Marti & Jaume Puig   Group: iGEM20_UPF_Barcelona   (2020-10-26)


Bad promoter + LuxI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 262
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 931
    Illegal BamHI site found at 202
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The pBAD was used to control the AHL expresion, as it can be induced by Arabinose or repressed by Glucose.


Source

All the parts were provided by Synthetic Biology for Biomedical Applications laboratory in our univeristy, UPF.

References